pFI Method for o-Phosphate
Room temperature, 120/40 s/hour, detection limit 6 ppB P
The flow programming comprises five sections within each assay cycle:
1. Priming @100%fr for 5 sec
2. Sample injection @50%fr for 1 sec
3. Incubation @2%fr for DWELL sec
4. Monitoring @50%fr for 12 sec
5. Washout @100% for 6 sec
The high ppB calibration range (A) was run at zero dwell time, while the low ppB range (B) was run using 50 seconds dwell time.
The overlaid calibration runs (C) show that as sampling frequency decreased from 120 s/hr to 40s/hr, the sensitivity of the assay increased almost 10 times, due to longer incubation time.
For all assays the injected sample volume was 300microliters and the flow cell light path was 10 cm. All manifold tubing was 0.8 mm I.D.
1.2.30.
